QBlueTM Cell Viability Assay Kit - 5000 assays

This homogeneous assay involves simply adding a single reagent, the QBlue reagent to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 - 570nm, emission wavelength = 590 - 620nm) after an incubation step. The QBlue reagent, like other resazurin-based assays, utilises the redox dye resazurin, which is not fluorescent, but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a fluorescence spectrophotometer or plate reader. Non-viable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The Qblue reagent has been optimised for maximum sensitivity, reproducibility and long shelf life. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Application:1. Cell Proliferation assays - effects of cytokines, growth factor and nutrients.2. Cytotoxicity and Apoptosis assays - evaluation of toxic compounds, anticancer antibodies and toxins.3. Drug Discovery - high-throughput screening for anti-cancer drugs. Method:EIA Species: Storage:Store at -20°C Stability:Stable up to 12 months from production. Sensitivity: Time: