Superoxide Dismutase Assay Kit - 200 µl

Superoxide Dismutase (SOD) catalyzes the dismutation of the superoxide radical (O2-) into hydrogen peroxide (H202) and elemental oxygen (O2), and as such, provides an important defense against the toxicity of superoxide radical. Overexpression of SOD protects murine fibrosarcoma cells from apoptosis and promotes cell differentiation. SOD also inhibits adriamycin-induced apoptosis in murine peritoneal macrophages. Superoxide ions, generated from the conversion of xanthine to uric acid and hydrogen peroxide by xanthine oxidase, converts NBT to NBT-diformazan. NBT-diformazan absorbs light at 550 nm. SOD reduces the superoxide ion concentration and thereby lowers the rate of NBT-diformazan formation. The extent of reduction in the appearance of NBT-diformazan is a measure of SOD activity present in your experimental sample. The assay is free of interference by other catalytic activities, and is ideal for assaying SOD in mammalian cell lysates. The kit contains the proper lysis buffer and the reagents needed for 100 experimental tests, 50 positive controls, and 50 negative controls. Unlike some other assay kits for SOD, this system is not greatly disturbed by trace metals. Each assay requires only about five minutes, and after a simple calculation, the percent inhibition of the formation of NBT-diformazan by SOD is converted to the relative activity of the sample. Application:Calculating SOD activity by measuring the reduction of NBT-diformazan in cell extracts. Method: Species: Storage:Store components at 4°C.