SREBP-2 Transcription Factor Assay Kit - 96 Well

Lipid homeostasis in vertebrate cells is regulated by a family of transcription factors called sterol regulatory element-binding proteins (SREBP`s). SREBP`s directly activate the expression of over 30 genes involved in the synthesis and uptake of cholesterol, fatty acids, triglycerides, and phospholipids. Three different SREBP isoforms designated SREBP-1a, SREBP-1c, and SREBP-2 are encoded by two different genes and belong to the basic helix-loop-helix-leucine zipper (bHLH-ZIP) family of transcription factors. SREBP-2 performs a critical role in the transcriptional regulation of genes involved in cholesterol synthesis and uptake including HMG-CoA synthase, HMG-CoA reductase, and the LDL receptor. Cayman`s SREBP-2 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized to the wells of a 96 well plate. SREBP contained in a nuclear extract, binds specifically to the SREBP response element. SREBP is detected by addition of specific primary antibody directed against SREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.Application/Transcription Factor Assay, Chemical Class/Assay Kits, Research Area/Atherosclerosis, Research Area/Metabolic Syndrome, Research Area/ObesitySynonyms :Sterol Regulatory Element-binding Protein-2 Assay Kit